The role of the morphologic categorization of p16INK4a/Ki-67 dual stained cytology in detecting of high grade cervical intraepithelial neoplasia (CIN2+).

Sopio Kakaliashvili-Dzagnidze, Omar Khardzeishvili, Sergo Tabagari, Teona Salukvadze



Objective: The aim of the study is to analyze the morphological characteristics of p16INK4a/Ki-67 dual stained cells (DS)  in cytology, to expand understanding of potential diagnostic value of integration of biomarkers in cytology and contribute to the existing knowledge on their utility in cervical cancer screening.

Materials and Methods: In our study included a total 162 woman, who had previous abnormal Pap screening results and were enrolled in an opportunistic screening program. For these participants 162 pap /p16INK4a/Ki-67 co- tests results and 29 histopathology results were available.

Results: In our study, sensitivity, specificity, PPV, NPV and accuracy of p16INK4a/Ki-67 DS of cervical smear, with abnormal morphology of stained cells to detect histologic high-grade cervical intraepithelial neoplasia (CIN2+) were 91%, 94%, 93%, 94% and 93%, respectively(


Key Words: p16INK4a/Ki-67 dual immunostaining (DS), CIN2+(CIN2 and CIN3) High-grade cervical Intraepithelial neoplasia; ASC-US, LSIL, HSIL, HR-HPV.

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