Preclinical models for oral squamous cell carcinoma investigations – review

Marika Zurmukhtashvili, Avtandil Machavariani, Giorgi Menabde, Ketevan Gogilashvili




In the last two decades, the recent advances and discoveries in genomic technologies have dramatically expanded our views and understanding of molecular genetic mechanisms and genetic anomalies underlying oral squamous cell carcinoma. Further experiments with relevant in vitro and in vivo experimental models are very important to gain deep knowledge of disease progression and therapeutic response. A relevant animal model is an essential key for understanding processes underlying the initiation and progression of oral cancer. There is a number of studies on different animals which can be used for models.


Therefore, this review aims to provide a reference and direction for researchers working in the field of oral oncology.


Data were collected using the electronic search method in the following databases: PubMed/Medline, Scopus. Terms “oral carcinoma”, AND “animal model” were utilized. English articles published between 2010-2019, with available summary were included.


Total 138 articles were found from different sources. Finally, 65 were selected for this review. After analysis and synthesis of information from articles, we have described different models currently used in oral oncology research and have provided details regarding their establishment and differences in their mechanisms, characteristics, and applications as well as regarding selection methods


Due to the characteristics of OSCC comprehensive understanding of model establishment is crucial for thorough investigations of these diseases. Unfortunately, it is impossible to completely replicate the natural situation of oral cancer. Every different model using different animals should be considered in regard to the field of planned studies. Generally, it was demonstrated that mice models can come most close.


oral cancer, cell line, animal model,

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