Dental Pulp Tissue Engineering

Abstract

The presented work discusses methods for creating dental pulp based on the principles of tissue engineering using decellularized scaffolds and bone marrow stem cells.

Materials and methods: Decellularized human dental pulp tissue, which was loaded with rat bone marrow stem cells, was used as a scaffold. The resulting bioengineered pulp was placed into a tooth section/framework, followed by implantation into the subcutaneous space of the rat's back.

Results: Research has shown that decellularized human pulp tissue loaded with bone marrow stem cells can be used as a scaffold for dental pulp bioengineering. Studies have shown that our bioengineered pulp, after heterotopic implantation, forms vascularized tissue similar to dental pulp and can express markers involved in dentinogenesis and angiogenesis.

Conclusions: Decellularized human dental pulp tissue has great potential for use as a cell carrier in tissue engineering.