TRANSLATIONAL AND CLINICAL MEDICINE - Georgian Medical Journal

Introduction: Many hepatic pathologies associated with the ductular reaction, changes of biliary mucosal glands, inflammation with leukocyte infiltration, etc. involve the liver portal tracts. The number of investigations indicates the role of portal tracts in the liver regeneration, innervation of the hepatic parenchyma; also, the portal tracts are important for surgeries aiming to treat the biliary atresia or conduct the liver graft transplantation.

To clarify the role of the portal tracts in development of various hepatic disorders, as well as successful implementation new interventions using the elements of a portal complex, requires translational investigations in different animals and therefore the studies of their portal tracts architecture for this purpose.

Method:We studied the architecture of the portal tracts of humans, canines and ratswith the following comparative analysis. The methods of macerating preparations,corrosion casts of tubular structures, histology (histotopography, histochemistry, immunohistochemistry) were used.

Conclusion: It was revealed that the overall architecture of the human, dog and rat portal tracts and the location of the elements in them is alike: the portal veins are surrounded by arteries and bile ducts, which are localized more peripherally – bordering liver parenchyma. However, in the animals the arterial-fibrous and biliary fibrous layers, which are the typical structures in humans, are less clearly developed.Humans’ and dogs’ bile ducts are supplied with mucous glands, while in rats, these structures are replaced by a periportal biliary plexus. This factshould be related to the absence of gallbladder and therefore, to the different conditions of bile drainage.

Kordzaia, D., Chanukvadze, I. & JM. Functional Anatomy of Intrahepatic Biliary System (Clinical and Experimental Data). In: Bile Duct: Functional Anatomy, Disease and Injury Classification and Surgical Management. Nova Science Publishers, Inc; 2014. p. 1–87.

Desmet V, Roskams T, Van Eyken P. Ductular reaction in the livers. Pathology-Research and Practice. 1995 Jul 1;191(6):513-24.

Roskams TA, Theise ND, Balabaud C, Bhagat G, Bhathal PS, Bioulac‐Sage P, Brunt EM, Crawford JM, Crosby HA, Desmet V, FinegoldMJ. Nomenclature of the finer branches of the biliary tree: canals, ductules, and ductular reactions in human livers. Hepatology. 2004 Jun;39(6):1739-45.

Moreira RK, Cabral R, Cowles RA, LobrittoSJ. Biliary atresia: a multidisciplinary approach to diagnosis and management. Archives of pathology & laboratory medicine. 2012 Jul;136(7):746-60.

Chanukvadze I. M. Structure and Connections of Fibrous Sheaths of Portal Complex and Hepatic Veins. Collections of Scientific Works of Tbilisi State Medical Institute., 1989, #1:11

Chanukvadze I, Archvadze V. Surgical anatomy of main intrahepatic portal tracts. Zentralblatt fur Chirurgie. 2003 Nov;128(11):958-62.

I.Chanukvadze, V.Archvadze, M.Soreli, G.Gudjabidze. Bilio-vascular architecture of main magistral portal tracts. Critical Care & Catastrophe Medicine. 2012; 1: 77-81

Dezső K, Rókusz A, Bugyik E, Szücs A, Szuák A, Dorogi B, Kiss M, Nemeskéri Á, Nagy P, Paku S. Human liver regeneration in advanced cirrhosis is organized by the portal tree. Journal of hepatology. 2017 Apr 1;66(4):778-86.

McCuskey RS. Anatomy of efferent hepatic nerves. The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology. 2004 Sep 1; 280(1):821-6.

Chaib E, RibeiroJr MA, Saad WA, Gama-Rodrigues J. The main hepatic anatomic variations for the purpose of split-liver transplantation. InTransplantation proceedings 2005 Mar 1 (Vol. 37, No. 2, pp. 1063-1066). Elsevier.

ChanukvadzeI.M. Construction and interactions of paravasal connective tissue structures in the liver. PhD thesis. Tbilisi State Medical Institute, 1979.

Sareli M, Chanukvadze I, Valeanu A, Zippel DB, Shapiro R, Papa MZ. The posterior intrahepatic approach to the left portal pedicle using the ligamentumvenosum: anatomical basis. Surgical and radiologic anatomy. 2009 Dec 1;31(10):809.

Beresford WA, Henninger JM. A tabular comparative histology of the liver. Archivumhistologicumjaponicum. 1986;49(3):267-81

Shogo Hayashi, Gen Murakami, Aiji Ohtsuka, Masahiro Itoh, Takashi Nakano And Yoshitaka Fukuzawa; Connective tissue confi guration in the human liver hilar region with special reference to the liver capsule and vascular sheath. J Hepatobiliary Pancreat Surg (2008) 15:640–647;

Lefkowitch JH. Special stains in diagnostic liver pathology. In Seminars in diagnostic pathology 2006 Aug 1 (Vol. 23, No. 3-4, pp. 190-198). WB Saunders).

Rauterberg J, Voss B, Pott G, Gerlach U. Connective tissue components of the normal and fibrotic liver. KlinischeWochenschrift. 1981 Jul 1;59(14):767-79.

Pott G, Rauterberg J, Voss B, Gerlach U. Connective tissue components of the normal and fibrotic human liver. KlinischeWochenschrift. 1982 Jan 1;60(1):1-7.